Experts give you examples to prove the reasons for the loss of ethylene oxide sterilization
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As we all know, ethylene oxide has strong penetrating power, wide sterilization spectrum, strong sterilization ability, no damage to sterilized items, and reliable sterilization effect. It is one of the commonly used low-temperature sterilization methods. A hospital used an ethylene oxide sterilizer for 50 sterilization operations, and the biological monitoring failed for 5 times, and the sterilization success rate was 90%. The reasons for the failure of ethylene oxide sterilization are summarized as follows.
The ethylene oxide sterilizer uses a single bottle of pure ethylene oxide to load the sterilized item package according to the regulations. Set the sterilization operation program parameters as sterilization temperature 50 ~ 58"C, sterilization time 60 ~ 180min, humidification time 2 ~ 10min. Start the sterilization program, the entire sterilization cycle is 240min. Each sterilization is monitored by the program .Place a chemical indicator card in each sterilized item package, and stick a chemical indicator tape outside the package as a sign of the sterilization process. Use the challenge package to monitor for the first time in ethylene oxide sterilization, and use the conventional test package to test for Ft normal sterilization.
The challenge test kit is to put a biological indicator in a 20rnl syringe, remove the needle and the needle cap, the plastic cap with the hole of the biological indicator should face the needle of the syringe, and then put the syringe core in place; another adult type is selected. Endotracheal intubation or a plastic syringe, an amber latex tube and 4 clean cotton surgical towels, folded into a challenge bag, then put the above items on the middle layer of the towel, and finally choose two cleaning cloths or non-woven wraps. Can. Arrange the test packs at 5 points on the front and rear of the upper layer of the sterilizer, the diagonal line, the center of the middle layer, and the front and rear diagonal lines of the lower layer. After the operation process is over, take out the biological indicator, squeeze the ampoule contained therein, and place it in a 37°C culture pot together with a control tube for cultivation; after 48 hours, read the result, the sterilization tube does not change color and turns green, indicating that the sterilization has passed; The sterilization tube changes color to yellow, indicating that the sterilization fails.
Updated：2021-02-25
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